Quote from: FDR101 on September 08, 2017, 06:56:39 PMDo you by any chance know if new soft tissue is created during LL around the bone in question, or is it simply stretched on a permanent basis?
For all I know
https://www.ncbi.nlm.nih.gov/pubmed/9167625
QuoteStudies of limb lengthening have demonstrated successful bone formation in the distraction gap. Failure of the muscle units to lengthen leads to many complications that significantly limit the success of this approach; it is, therefore, of paramount importance to characterize the behavior of the muscle during limb lengthening. In this study, tibiae of adult rabbits were lengthened for 10 days at a rate of 1 mm/day. The proliferative ability of the lengthened muscle was characterized using bromodeoxyuridine, a thymidine analogue that is incorporated during cell division, and desmin, a muscle-specific marker. We observed a large number of proliferating cells, specifically in the lengthened muscle, that were co-localized with many desmin-positive cells. The presence of bromodeoxyuridine nuclei inside desmin-positive muscle fibers suggests that limb lengthening promotes muscle growth by triggering myoblast proliferation and fusion into the lengthened muscle. Our findings are consistent with those of other studies in the reviewed literature that also suggest that limb lengthening promotes muscle growth.
https://www.ncbi.nlm.nih.gov/pubmed/10672601
QuoteExperimental animal studies and clinical investigations show three processes going on during extremity lengthening with the Ilizarov-method. At first degenerative changes including cell necroses in the muscles, the nerves and the tendons [corrected] occur as well as denervation of muscle fibers resulting in neurogenous muscle atrophies. These alterations are followed by reperative and regenerative processes as well as the reinnervation of the denervated muscle fibers. Secondly histoneogenesis occur which leads to a high increase of tissue-specific cells and a growth of the muscles, tendons and vessels. Therefore tensile-stress is an important factor of tissue growth. Thirdly adaptive processes proceed during leg lengthening. For example the high biosynthetic activity during tissue growth seems to indicate an increase of vasa vasorum.
https://www.ncbi.nlm.nih.gov/pubmed/23049683
QuoteSkeletal muscle responds to passive overstretch through sarcomerogenesis, the creation and serial deposition of new sarcomere units. Sarcomerogenesis is critical to muscle function: It gradually re-positions the muscle back into its optimal operating regime. Animal models of immobilization, limb lengthening, and tendon transfer have provided significant insight into muscle adaptation in vivo. Yet, to date, there is no mathematical model that allows us to predict how skeletal muscle adapts to mechanical stretch in silico. Here we propose a novel mechanistic model for chronic longitudinal muscle growth in response to passive mechanical stretch. We characterize growth through a single scalar-valued internal variable, the serial sarcomere number. Sarcomerogenesis, the evolution of this variable, is driven by the elastic mechanical stretch. To analyze realistic three-dimensional muscle geometries, we embed our model into a nonlinear finite element framework. In a chronic limb lengthening study with a muscle stretch of 1.14, the model predicts an acute sarcomere lengthening from 3.09[Formula: see text]m to 3.51[Formula: see text]m, and a chronic gradual return to the initial sarcomere length within two weeks. Compared to the experiment, the acute model error was 0.00% by design of the model; the chronic model error was 2.13%, which lies within the rage of the experimental standard deviation. Our model explains, from a mechanistic point of view, why gradual multi-step muscle lengthening is less invasive than single-step lengthening. It also explains regional variations in sarcomere length, shorter close to and longer away from the muscle-tendon interface. Once calibrated with a richer data set, our model may help surgeons to prevent muscle overstretch and make informed decisions about optimal stretch increments, stretch timing, and stretch amplitudes. We anticipate our study to open new avenues in orthopedic and reconstructive surgery and enhance treatment for patients with ill proportioned limbs, tendon lengthening, tendon transfer, tendon tear, and chronically retracted muscles.
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